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Identifying the optimal root system architecture (RSA) for Brassica crops

The current project aims to define a root system architecture RSA that contributes to improved NUE for canola and will allow the reduction of nitrogen inputs while maintaining productivity. With increasing temperatures predicted for the Prairies in coming years it is becomes imperative to generate climate resilient crops.

Defining populations of Plasmodiophora brassicae with near isogenic Brassica napus lines

The differential lines will provide canola pathologists and breeders with an extremely valuable tool for assessing the effectiveness of resistance. They will be made available to the canola industry for variety development, which will ensure that Saskatchewan producers have a diverse range of clubroot resistant cultivars to select from.

Identification and genetic mapping of novel genes for resistance to blackleg in Chinese and Canadian Brassica napus varieties

Genetic resistance is considered as the most efficient method for control of blackleg. Previous research results indicate that both Canadian and Chinese B. napus varieties could carry novel genes for resistance to blackleg. Therefore, it is necessary to identify and map the unknown R genes in the canola varieties.

Manipulating recombination in crop polyploids

There is a pressing need to improve productivity of crops, in order to maximize yield without further expanding arable land. The ability to make further crop improvements relies on the introduction of novel allelic variation, one such source being related species; however, interspecific barriers to recombination limit the transfer of new variation into crops.

Addressing yield stability drivers of canola in a changing climate using high throughput phenotyping

The tools developed and verified through this project will enable efficient development of resilient varieties. The results support potential of canola digital phenotypes to field-scale agronomic applications. The expansive data sets and samples generated through this project are and will be used in various research projects, extending the utility of grower-invested research dollars.

Pre-breeding lines combining canola quality with sclerotinia resistance, good agronomy and genomic diversity from PAK93

Seed of PAK93-based pre-breeding lines will be more attractive to plant breeding companies than the original germplasm such as PAK54, primarily because it will be faster to develop hybrid varieties that combine the desirable traits from PAK93-derived lines with other important traits such as herbicide tolerance and resistance to the diseases, blackleg and clubroot.

Enabling Canola Protein Optimization (ECPO)

Increase canola protein inclusion rates in monogastric animal feeds, followed by canola germplasm that produces protein better suited for human diets, and finally specialty varieties that produce protein for specific technical applications.

A proteomics-based approach towards identifying host and pathogen proteins critical to clubroot establishment in canola

The proposed research will deliver knowledge and tools to improve utilization of existing clubroot resistant cultivars and to accelerate the discovery of new clubroot resistance genes, with the anticipation of exploring broad-spectrum and durable clubroot resistance that will be highly beneficial to breeders and growers of canola and other Brassica crops.

Cloning clubroot resistance genes from B. nigra and transferring the genes into canola through a CRISPR/Cas9 based technology

The pathogen (P. brassicae) populations in western Canada is evolving rapidly. It is extremely important to have canola cultivars with new sources of resistance avialabe to canola producers in Saskatchewan.

Developing allele specific molecular markers for the B.napus blackleg resistance (Rlm) genes

Our knowledge of pathogen virulence genes and plant race specific resistance (R) genes in the Leptosphaeria-Brassica pathosystem has tremendously advanced in the past two decades.

Functional validation of Brassica napus genes related to clubroot resistance through high-throughput CRISPR/Cas9 genome editing

Growing clubroot-resistant (CR) cultivars in appropriate rotations remains the most effective solution to the long-term management of clubroot disease.

Understanding the molecular basis of NLR-mediated clubroot resistance in Brassica napus

The ability to integrate stable clubroot resistance into new germplasm is needed to protect the economy while ensuring sustainability and growth in canola growing regions.